Cytokine Analysis Services for Cellular Communication

Cytokines are essential proteins that play a crucial role in cellular communication and immune function. Quantifying cytokine levels within biological samples is pivotal for various applications, including inflammation, infection, and cancer research. This web page provides an overview of cytokine testing services and their significance in assessing cellular communication.

Cytokine Testing Methods: There are several methods available for measuring cytokine levels, each with its own advantages and considerations:

  1. Enzyme-Linked Immunosorbent Assay (ELISA):

    • ELISA is a widely used method for assessing cytokine levels.
    • It is cost-effective but may be time-consuming and requires specialized equipment.
    • Suitable for single-cytokine analysis.

  2. Luminex Assay:

    • Luminex is a multiplex approach that can simultaneously measure multiple cytokines.
    • It offers high sensitivity and speed compared to ELISA but requires specialized equipment.
    • Ideal for studies requiring the analysis of multiple cytokines.

  3. Quantitative PCR (qPCR):

    • qPCR measures mRNA levels of cytokines, providing heightened sensitivity.
    • It offers higher sensitivity than ELISA or Luminex but is costlier and requires specialized equipment.
    • Best suited for research requiring gene expression analysis of cytokines.

Choosing the Right Method:
The choice of cytokine testing method depends on specific test requirements and resource availability. Consider factors such as the number of cytokines to be measured, sensitivity, speed, and cost when selecting a method.

Cytokine Testing Services: 
Cytokine testing services are available through various laboratories, including commercial, hospital, and research facilities. The cost of cytokine testing may vary depending on the laboratory, the type of test, and the number of cytokines to be measured.

Services Available at Jivakana Cell Technologies:
In addition to cytokine testing, Jivakana Cell Technologies offers a range of services, including:

  • SDS-PAGE
  • Western Blot
  • UV Spectrophotometry (A280)
  • Sterility per USP <71>
  • Endotoxin per USP <85>

For more detailed information on cytokine testing, you can refer to the FDA Guidance for Industry: Guidance for Human Somatic Cell Therapy and Gene Therapy.

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